Alsuhaymi N
.Neuropharmacology and Neuroinflammation, School of Health and Biomedical Sciences, RMIT University, Melbourne, VIC, Australia
Title: The effect of central administration of resistin or leptin alone and in combination on the activated catecholaminergic, serotonergic and orexinergic neurons in the brain of rats fed a normal diet vs. high fat diet
Biography
Biography: Alsuhaymi N
Abstract
Adipose tissue plays a critical role in the regulation of endocrine and cardiovascular function by secretion of bioactive peptides called adipokines (e.g. Resistin, and leptin) that can act centrally to influence these functions. Resistin, and leptin have roles in cardiovascular function, feeding and energy balance; and can act in the brain nuclei known to be important for mediating these functions. Central sites of action for resistin are not well understood, in contrast to leptin. Moreover, in high fat diet (HFD), resistin and leptin are increased, but little is known regarding the interactions between these hormones in the brain. This work investigated the neurochemical content of the neurons activated by resistin and leptin alone or in combination. In this work the rats were divided into two groups with each group receiving either a HFD or a normal diet. Anaesthetized rats received ICV of saline (control), resistin or leptin alone or in combination. Three hours later, the brains were perfused and dual-label immunohistochemistry was performed to detect Fos protein and tyrosine hydroxylase (TH, for catecholamines) or tryptophan hydroxylase (for serotonin) or orexin. In conclusion, following resistin and leptin ICV, there was activation of TH neurons in the PVN, NTS and RVLM and orexinergic neurons in the LHA. A HFD can reduce the sensitivity of TH neurons in the PVN and orexinergic neurons in the LHA. Thus, increased dietary fat can influence the responsiveness of some CNS pathways to resistin as well as leptin.